A new Phenol Red-modified porphyrin as efficient protein photocleaving agent.

نویسندگان

  • Guo-Yu Jiang
  • Wan-Hua Lei
  • Qian-Xiong Zhou
  • Yuan-Jun Hou
  • Xue-Song Wang
  • Bao-Wen Zhang
چکیده

Protein affinity is of importance for porphyrins in their application in photodynamic therapy (PDT). A new Phenol Red-modified porphyrin (R-TPP) was designed and synthesized to fully take advantage of the binding character of Phenol Red towards protein. Detailed comparisons of absorption spectra, fluorescence spectra, n-octanol/water partition coefficients, (1)O(2) quantum yields, as well as protein photocleaving abilities between R-TPP and its parent porphyrin Br-TPP clearly demonstrate the benefits stemming from the modification of Phenol Red. On one hand, the presence of Phenol Red moiety greatly enhances the binding affinity of R-TPP towards model proteins (bovine serum albumin and hen egg lysozyme), and therefore improves the availability of (1)O(2). On the other hand, the presence of Phenol Red moiety provides R-TPP with amphiphilic character, and therefore restricts aggregation and favors the generation of (1)O(2). As a result, R-TPP photocleaves proteins efficiently, showing promising application potential in PDT.

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عنوان ژورنال:
  • Physical chemistry chemical physics : PCCP

دوره 12 38  شماره 

صفحات  -

تاریخ انتشار 2010